Vibrio vulnificus에서 lipopolysaccharide(LPS)와 cell wall protein A를 추출하여 치사독성 및 Limulus겔화 활성을 측정한 후 LPS의 효소활성에 미치는 cell wall protein A의 영향을 조사하였다. 이 결과들을 Escherichia coli와 Salmonella typhimurium LPS 및 cell wall protein A의 것들과 비교하였다. V. vulnificus LPS의 치사독성($LD_{50}$는 138.6 mg/kg)은 E. coli LPS와 S. typhimurium LPS의 것(56.3 mg/kg와 37.5mg/kg)보다 낮았지만 세가지 LPS의 Limulus 활성은 모두 같았다(0.1 ng/ml). LPS와 cell wall protein A에 의한 alanine aminotransferase(ALT), aspartate aminotransferase(AST)와 lactate dehydrogenase(LDH)의 활성은 증가하였다. 한편 LPS의 효소활성에 미치는 cell wall protein A의 영향은 커서 LPS와 cell wall protein A를 혼합한 것의 효소활성이 LPS만의 것보다 컸다.
Lipopolysaccharide (LPS) and cell wall protein A from Vibrio vulnificus were extracted, were tested the lethal toxicity and Limulus amebocyte gelation activity, and were examined the effect of cell wall protein A on the enzyme activity of LPS. These results were compared to those of LPSs and cell wall protein As from Escherichia coli and Salmonella typhimurium. The lethal toxicity of V. vulnificus LPS ($LD_{50}$ was 138.6 mg/kg) was lower than those of E. coli LPS (56.3 mg/kg) and S. typhimurium LPS (37.5 mg/kg), but the Limulus gelation activities of all LPSs were the same value (0.1 ng/ml). The results of enzyme activity by LPS and cell wall protein A have shown that the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) were increased. The cell wall protein A on the enzyme activities of LPS was effective; the enzyme activities of LPS-cell wall protein A mixture were greater than those of LPS only.
