BPTI(Basic Pancreatic Trypsin Inhibitor)에 phase-sensitive COSY(shift COrrelation SpectroscopY), DQF(Double Quantum Filtered)-COSY, TOCSY(TOtal shift Correlation SpectroscopY), NOESY(two-dimensional NOE SpectroscopY) 등의 2차원 핵자기공명분광법을 사용하여 수소 공명주파수를 지정하고 $^{3}J_{HNa}$ 값을 측정하여 기존 문헌치(Wagner et al., 1987; Berndt et al., 1992)와 비교한 결과 chemical shift 값, 공명주파수 지정에 사용된 순차적 NOE 및 $^{3}J_{HNa}$ 값 등이 거의 일치한다. 한편, 본 연구에서는 기존 문헌에서 지정하지 않은 14개의 수소 공명주파수를 추가로 지정할 수 있었다.
Two-dimensional NMR methods such as phase-sensitive COSY (shift COrrelation SpectroscopY), DQF (Double Quantum Filtered)-COSY, TOCSY (TOtal shift Correlation SpectroscopY), NOESY (two-dimensional NOE SpectroscopY) in $H_{2}O$ and $D_{2}O$ have been used to achieve resonance assignment for basic pancreatic trypsin inhibitor. Results are in good agreement with the previous reports by Wuthrich and his coworkers (Wagner et al., 1987; Bemdt et al., 1992). In addition, in this work, we have assigned 14 additional resonances that were not assigned before.
