This study was aimed to evaluate the anti-stress effect of Chungkansoyosan on the rats stressed by immobilizaton. The experimintal animals were immobilized in stress box $(5cm{ imes}5cm{ imes}20cm)$ for 12 hours in a day during 3 days, and administered 90ng/100g Chungkansouosan extract for 15 days before stress. The epinephrine, dopamine contents in rat brain were measured by using HPLC method. The following results were obtained: 1. Total cholesterol contents of control group was $58.1{pm}2.56mg/dl$, that of sample group was $61.6{pm}3.70mg/dl$. This shows decreasing effect, but not significant. 2. Triglyceride contents of control group was $39.8{pm}6.75mg/dl$ that of sample group was $27.7{pm}4.23mg/dl$. This shows significant decrease in sample group comparing to control group. 3.Epinephrine contents of control group was $8.21{pm}0.31ng/mg$ that of sample group was $6.33{pm}1.02ng/mg$ this shows significant decrease in sample group comparing to control group. 4. Dopamine contents of control group was $2.45{pm}0.23ng/mg$ that of sample group was $1.65{pm}0.41ng/mg$ this shows decreasing effect, but not significant. 5. The quantity of muscle relaxation by Rota Rod was improved significantly by Chungkcansoyosan in immobilizaton-stressed rats. According to the above results, it is concluded that Chunkansoyosan will be useful as a preventive and remedy against stress disease.