The combustion characteristics of diesel oil and fish oil blended with diesel oils were investigated at various blending rate of fish oil in diesel engine and constant volume combustion bomb. The evaporation and combustion duration of diesel oil and fish oil blended with diesel oils were respectively different high and low temperature. The dependence of ignition delay on the temperature was different in high and low temperature ranges which were divided at the 773K. The ignition delay become longer than that of diesel oil as the blending rate of fish oil increases, and its difference were larger at different loads. The densityof smoke was lower as the blending rate of fish oil increases, and the rate offuel consumption showed no significant difference between diesel oil and fish oil blended with diesel oils.