미국흰불나방의 성숙란에서 추출한 난황단백질 1,3(yp-1, yp-3)(Lee et al., 1995)의 항체를 이용하여 이 단백질의 합성장소인 지방체와 난모세포에서 흡수, 저장과정을 전자현미경을 이용한 금입자 면역표지법 (electronmicroscopic immune-gold labelling)으로 살펴보았다. 난황형성이 시작되기 직전의 지방체세포에 존재하는 단백질과립들에서 yolk protein항체에 의한 면역반응이 관찰되었고, 난황형성이 시작되면 난소소관 초(sheath), 미세음세포 소포들, 그리고 난황과립 등에서 양성 반응이 나타났다. 난황형성이 활발할수록 여포세포간 간극은 넓어지면서 난모세포막에서의 난황전구물질의 흡수도 활발해졌다. 단백질성 난황구의 형성은 작은 소포들이 서로 융합되어 커다란 막구조물을 형성한 다음 여기에 난황단백질 1, 3를 포함한 여러가지 단백질이 채워지고 있었다.
The yolk protein synthesis in the fat body and uptake on storage processes in ovary were studied by electronmicroscopic immunogold - labelling method, using polyclonal antibody against yolk protein 1(yp-1) and yolk protein 3 (yp-3) (vitellogenin) from mature eggs of Hyphantria cunea. Gold particles were immunosensitively labelled on protein granules in fat body cells, in that stage which it was not yet observed the yolk formation in the ovary. As the yolk formation began, gold particles were altenatively labelled on the ovarian sheath, oocyte membrane, micropinocytotic vesicles, and yolk granules. During the yolk formation, the intercellular space between follicle cells became wider and yolk proteins were more actively absorbed into the oocyte. Noticeably, in order fur formation of the large proteoid yolk spheres, micropinocytic vesicles fused together into a vacant large membraneous sphere and then filled with several Proteins, including yp-1 and 3.
