Tri-phasic pulsatile flow is the general flow pattern of human circulary system. In emulating the various situation of cardiovascular system, it is essential to make tri-phasic source flow. To emulate tri-phasic pulsatile flow, we used electro-hydronic style LVAD(Left Ventricular Assist Device) with glass phantom and rubber tube. We have selected control parameters and examined the changing effect of each parameter by using Doppler ultrasound. In this experiment, it was shown that the distal compliance and the break time were the major factors to form tri-phasic flow. The results make it possible to emulate and explain the various situation of human vascular system. In this point, this results will be an useful method in the clinic application.