연두금파리(Lucilia illustris)의 발생단계에 따른 20-hydroxyecdysone과 ecdysone의 농도변화를 high performance liquid chromatography (HPLC)법으로 측정하였다. ecdysone의 농도는 번데기 3일에서 64.58ng/g으로 가장 높은값을, 종령유충 배회기에서 가장 낮은값(0.99ng/g)을 나타냈다. 반면 20-hydroxyecdysone의 농도는 흰번데기에서 75.46ng/g으로 최고값을, 번데기 7일에서 10.50ng/g으로 최저값을 나타냈다. 유충-번데기시기에 20-hydroxyecdysone농도변화는 섭식을 중지하고, 형태적 특징으로 노출된 심장 및 분홍색 복부선을 보이는 종령유충 배회기 그리고 흰번데기에서 2개의 peaks를 관찰했다. 전 발생단계에 따른 20-hydroxyecdysone은 ecdysone보다 활성형으로 나타났으며 20-hydroxyecdysone의 농도는 ecdysone의 농도와 상대적 역관계를 보여 ecdysone이 효과적인 저해능으로 나타났다.
Variations in the 20-hydroxyecdysone and ecdysone was analyzed by high performance liquid chromatography (HPLC) during the development of the blowfly, Lucilia illustris. The concentration of ecdysone was the maximum (64.58 ng/g) at day-3 pupa and minimum (0.99 ng/g) at wandering larva, but 20- hydroxyecdysone was peaked at the white pupa (75.46 ng/g) and declined to the minimum (10.50 ng/g) at day-7 pupa. The late third instar larva ceased feeding and became wandering larva, which were morphologically characterized by an exposed heart and the appearane of a pink pigment along the dorsal midline. During this period, a smaller increase in the titer was observed at wandering larva and was the increase presumed responsible for commitment to pupal development. For the large peaks preceeding larval and pupal ecdysis, 20- hydroxyecdysone was the predominant hormone, while ecdysone was the major component during early pupa development. In conclusion, ecdysone and 20- hydroxyecdysone titer was shown to be inversely proportional.
