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Hyper-CMCase-Producing Mutants of Bacillus sp. 79-23 Induced by Gamma- Radiation
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  • Hyper-CMCase-Producing Mutants of Bacillus sp. 79-23 Induced by Gamma- Radiation
  • Hyper-CMCase-Producing Mutants of Bacillus sp. 79-23 Induced by Gamma- Radiation
저자명
Yoon. Ki-Hong,Shin. In-Kyung,Jung. Kyung-Hwa,Park. Seung-Hwan
간행물명
Journal of microbiology and biotechnology
권/호정보
1999년|9권 4호|pp.518-521 (4 pages)
발행정보
한국미생물생명공학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Bacillus sp. 79-23 spores were irradiated with $^{60}Co$ gamma-rays at doses ranging from 0.5 to 5 kGy. Following gamma-irradiation, seven mutant strains were isolated by scoring the halo sizes formed around the colonies grown on LB agar plates containing 4% carboxymethylcellulose (CMC) and trypan blue. The mutant strains showed a 1.5 to 2-fold increase in carboxymethylcellulase (CMCase) activity over the parent strain. Wheat bran acted as an effective inducer for CMCase production in the parent and mutant strains. Mutant strains 68 and 70 were identified as exhibiting higher CMCase activities than those of other mutants in LB media both with and without 3% wheat bran. In addition, these strains seem to produce substantially lower amounts of capsular materials, whereas the parent strain produced large amounts of them in both liquid and solid LB media. In flask cultures, the CMCase production by mutants 68 and 70 reached maximum levels of 17.5 unit/ml and 15.7 unit/ml, respectively, in an LB medium containing 3% wheat bran.