황복 (Takifugu obscurus)의 정자보존을 위한 기초자료를 얻고자, 정액의 특성을 파악하고 정자의 냉장보존 조건에 관한 연구를 수행하였다. 정액의 ml당 정자수는 $1.13pm0.34 imes10^{10}/ml$이었고 spermatocrit는 64.8$pm$1.4였으며, 정장의 삼투질농도는 266$pm$2 mOsm/kg이었다. 총 단백질 함량과 총 지질 함량은 정장에 비해 정자에서 높았으며, glucose는 검출되지 않았다. $Ca^{2+}$과 $Na^{+}$ 농도는 정자에 비해 정장에서 높았고, $Mg^{2+}$과 $K^{+}$은 정장에 비해 정자에서 높은 값을 보였다. 황복 정자를 여러 가지 희석액을 이용하여 16 일간 $0pm0.5^{circ}C$에서 냉장보존 하였을 때, 수정률은 $0~0.7\%$로 보존 효과가 저조한 것으로 나타났다.
To obtain the basic data for the preservation of river puffer (Takifugu obscurus) sperm, experiments were carried out on the physico-chemical properties and cold storage of milt. The average number of sperm and spermatocrit in milt stripped were $1.13pm0.34 imes10^{10}/ml$ and 64.8$pm$1.4, respectively. Osmolality of seminal fluid was 266$pm$2 mOsm/kg, Total protein and total lipid from sperm were higer than that from seminal fluid. $Ca^{2+}$ and $Na^{+}$ concentrations were higher in the seminal fluid than in the sperm, while $Mg^{2+}$ and $K^{+}$ concentrations were lower in the seminal fluid. When sperm of river puffer were preserved in $0pm0.5^{circ}C$ with various diluents for 16 days, fertilization rate was $0~0.7\%$. It suggested that cold storage of river puffer sperm was detrimental to sperm fertility.
