The main objective of this work is to investigate the effect of ambient conditions on the spray behavior and spray characteristics of high-pressure fuel injector. For this purpose, the effects of ambient pressure and temperature on the spray characteristics have been studied by applying the analysis of visualization system and phase Doppler particle analyzer. In this experiment, the visualization of spray behavior was performed under various ambient gas conditions and injection parameters such as gas temperature, ambient pressure, injection pressure of injector, and axial distance from the nozzle tip. Based on the investigation results, the spray tip penetration and spray width decrease with the increase of ambient gas pressure in the spray chamber. The effects of the spray parameters on the microscopic characteristics of gasoline spray were discussed.