It is a serous subject for energy conservation to prevent the energy loss caused by the mixture of heated and cooled air jets in perimeter and interior zone of a building operated with tow kinds of air-conditioning system simultaneously. The purpose of this paper is to clarify the quantitative and qualitative mechanisms of mixing loss and to propose a evaluation method for it. By using the dynamic heat load calculation, heat extraction load of a typical office building in Busan are calculated. According to the results, numerical simulations based on CFD(Computational Fluid Dynamics) were performed in order to evaluate mixing loss in the physical size of HVAC system. Then, the distributions of air temperature and airflow patterns according to the differences of set-point temperature are analyzed to grasp relations how to influence mixing loss.