A well defined electro-spraying and electro-static precipitator(ESP) experiment is carried out to investigate the charging characteristics of the submicron particles and the monodisperse particles. The basic idea is that the highly charged electro-sprayed droplets will be produced into the gas when the Coulombic repulsive force on the surface is higher than the surface tension of the spraying liquid. During this process many highly charged smaller droplets or ions, if the droplets are completely dried out, will be produced in the space. These charged species will be attached ion the particles and then eventually charge the particles. These charged particles will be easily collected with ESP. The experimental results show that the atomizer generated particles with geometric mean diameter (GMD) of 62nm are charged more than 90% even at the mean face velocity of 2.5m/s at the charging zone.