The vortex tube is a simple device which separates fluid stream into a cold stream and a hot stream without any chemical reaction. The process of energy separation in the vortex tube has caused a great deal of interest. Although many studies on energy separation in the vortex tube using air as the working fluid have been made so far, few experimental studies treated energy separation for incompressible fluid. So, an experimental study for the energy separation in the vortex tube using the water which is essentially an incompressible fluid is presented. When working fluid is the water, the best geometric values of nozzle area ratio and number of nozzle holes are 0.155, 6 respectively. These geometric values are showed by the similar values which are presented by compressible fluid as working fluid. But hot side mass fraction of which maximum temperature drop is happened are different from compressible fluid.