The authors have studied the 8 weeks clinical experiment on 34 dental patients of age 20s for control group and 32 for experimental 1 group(Paste Type Dentifrice with Bamboo Salt, Chitosan and Powder of Pine Needles and Leaves of Bamboo.) and 32 for experimental 2 group(Powder Type Dentifrice with Bamboo Salt, Chitosan and Powder of Pine Needles and Leaves of Bamboo), in order to find out the effect of plaque removal, prevention of calculus and stain formation. and Gingival effect. The obtained results are as following. 1. Plaque removal effect was the similar level on group 1 and on group 2 as control group. 2. For prevention of calculus formation, there was a little bit better on group 1 at 8 weeks, and for prevention of stain formation, it revealed more or less difference between on group 1, group 2 and control group at 8 weeks. 3. on PMA index, it revealed the significantly differences between group 1, group 2 and the control group at 8 weeks(p<0.05), so it is estimated that there might be signified for gingival subside effect by use of dentifrice with Bamboo Salt, Chitosan and Powder of Pine Needles and Leaves Bamboo.