This paper analyzes the subsurface stress at the spherical contact using Hamilton equation, and with that data, calculates the fatigue limit under the variations of friction coefficient using fatigue theory. After rough surface being made, this paper figures out the random load generated by contacting to the rough surface, analyzes the stress of its subsurface, and calculates the fatigue limit of the rough surface using fatigue theory. The three parts of the fatigue theory are applied, which are critical plane theory, stress invariant theory and mesoscopic theory.