The phenomena of energy separation in vortex tubes was investigated experimentally to see the subsidiary effect of the conductivity of tube material and cooling conditions around the outer surface of the tube. The experiment was carried out with pyrex, stainless steel and copper tubes, and the heat transfer conditions of the tubes were with insulation, without in-sulation and water cooling modes respectively The results were obtained that the hot exit fluid temperature was highly affected by a change of conductivity of a tube when the outer surface was cooled by the water, while the working fluid through the tubes was air. How-ever, the cold exit temperature was little affected by the heat transfer modes on the outer surface of the vortex tube.