This is the second of two part series on experimental studies of grout type transverse joints. In this paper, grout-type transverse joints between precast concrete slabs are tested to study the fatigue behavior. The tests are per-formed with loading equipment designed and constructed especially in the lab to introduce shear fatigue failures on the joints of the test specimens with repeated loads. Non-prestressed as well as prestressed specimens are selected based on static tests and these specimens are studied to identify the effect of prestress on the fatigue strength of the grout type joint. A comparison between prestressed and non-prestressed specimens indicates that longitudinal prestressing is an effective method to increase fatigue strength of the transverse joints. Based on the fatigue test, a rational estimation of the fatigue strength is proposed to aid design of the grout-type transverse joints.