A highly interferent-resistive membrane, poly-5-amino-1-naphthol (poly-5A1N), underlied beneath enzymeembedded poly-1,3-phenylendiamine (poly-m-PD) network for miniturized continuous monitoring glucose sensors. The enzyme layer was prepared from a mixed solution of glucose oxidase (GOx) and m-PD monomer by simple electrolysis. The mass change of poly-5A1N was monitored by electrochemical quartz crystal microbalance (EQCM) in situ and the corresponding thickness was measured. Successive electropolymerization of poly-5A1N and poly-m-PD create a several tens nm-thick bilayer showing excellent selectivity for $H_2O_2$ and low activity loss of immobilized enzymes.