In heavy nuclear power plant, high energy through main steam line is provided to turbine that generate the electric power. Since plant had generated power, high noise has been occurred. Noise make equipments and work environment worse. For finding out the location and the cause of making noise, noise was measured along main steam line at open/close test of Main Steam Isolation Valve (MSIV hereafter). As the result, it was identified that the vortex shedding in the cavity of MSIV is main noise source. The profile change of MSIV seat ring was proposed as the method of noise reduction. After filletting MSIV seat ring, the noise level reduced $10{sim}20dB$ compared before the change of profile.