This study investigated effect of arm posture, repetition of wrist motion and external load on perceived discomfort. The arm postures were controlled by shoulder flexion, elbow flexion, and ist motions such as flexion, extension, radial deviation and ulnar deviation. An experiment was conducted to measure discomfort scores for experimental treatments using the magnitude estimation, in which the L16 orthogonal array was adopted for reducing the size of experiment. The results showed that while the effect of the shoulder flexion, repetition of wrist motion and external load was statistically significant at $alpha=0.05$or 0.10, that of the elbow and wrist motions was not. Discomfor ratings increased linearly as levels of wrist repetition and external load increased. This implies that the existing posture classification schemes such as OWAS, RULA, which do not properly consider effect of motion repetition and external load, may underestimate postural load. Based on the regression equation for wrist repetition and external load, isocomfort region indicating the region within which discomfort scores were expected to be the same was proposed. It is recommended that when assessing risk of postures or developing new posture classification schemes, motion repetition and external load as well as posture itself be fully taken into consideration for precisely evaluating postural stress.