The antibiotic reactive dye was synthesized by coupling of reactive chromagen with diazotised silver sulfadiazine for an antibiotic property. The highly reactive MCT(monochlorotriazine) and DCT (dichlorotriazine) type functional groups which have heterocyclic ring and moderately reactive VS-type dye that has good dyeability were used for reactivity. The synthesized antibiotic reactive dye is expected to impart the antibiotic function with high durability on cotton fabric only by one-step dyeing process without further finishing treatment. The synthesis of antibiotic dye was easily proceeded thorough diazotisation of silver sulfadiazine and coupling with suitable chromogen. The dyeability of synthesized dye for cotton fabrics was excellent and the dyed fabrics showed good level of lightfastness, resistance to washing and rubbing. The antibiotic tests revealed that the dyed cotton fabrics with the synthesized dye had very good antibiotic properties.