The purpose of this study was to compare the postural muscle activity during wearing a lead apron with and without applying waist belt at working posture. Ten healthy male subjects were recruited for this study. Electromyography using a surface EMG recorded the activity of the splenius capitis, trapezius, and erector spinae. EMG activity was recorded at quiet standing, 45 degrees of neck flexion, 45 degrees of neck flexion with 15 degrees of trunk flexion. The testing order was selected randomly. The subjects were asked to maintain the each posture for 3 minutes. The mean root mean square (RMS) of EMG activity was calculated. EMG activity was normalized using the maximum voluntary isometric contraction (MVIC) elicited using a manual muscle testing technique. Two-factor repeated measures analysis of variance (ANOVA) was used to compare the average RMS value of EMG activity for each condition. The EMG activity of trapezius muscle was significantly decreased with applying waist belt (p<.05). The muscle activity of splenius capitis and erector spinae showed significant difference according to postures (p<.05). These results suggest that applying waist belt during wearing a lead apron will be useful to prevent shoulder pain.