The purpose of this study was to find the difference in muscle firing rate between each muscle according to the knee angle with the quadriceps femoris which is a representative action muscle of the lower extremity. Seven normal healthy subjects were recruited. The median frequency (MDF) of muscle contraction was recorded from vastus lateralis, vastus medialis, and rectus femoris muscles using the surface EMG, in 5 seconds, during maximal isometric knee extension. The data were analyzed by the two-way repeated ANOVA. The results of the study were as follows: 1) median frequency of muscle contraction was significantly higher at the vastus lateralis, vastus medialis, and rectus femoris in descending order. 2) median frequency of muscle contraction was significantly higher at the $30^{circ}$, $60^{circ}$, and $90^{circ}$ in descending order. Consequently, muscle recruitment at the knee decreases the EMG activity of the lengthened muscle. This study suggests that the change in EMG activity at different muscle lengths resulted in affecting the muscle firing rate during the knee extension.