- RT-PCR과 ELISA를 이용한 PRRS 진단 및 항체가 조사
- ㆍ 저자명
- 추금숙,한규삼,한재철,송희종,Chu. Keum-Suk,Han. Keu-Sam,Han. Jae-Cheol,Song. Hee-Jong
- ㆍ 간행물명
- 韓國家畜衛生學會誌
- ㆍ 권/호정보
- 2004년|27권 3호|pp.273-280 (8 pages)
- ㆍ 발행정보
- 한국가축위생학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
The studies were performed for the PRRS antigen and antibody detection from breeding farms, artificial insemination(AI) center and growing farms in Jeonbuk province. 1. Specific PRRS primers were successfully amplified ORF6 617bp and ORF7 448 bp on agarose gel. 2. RT-PCR method has been establish by commercial kit and the thermal cycler program consisted of 30 cycles: $95^{circ}C$ for 30 sec, $45^{circ}C$ for 30 sec, and $72^{circ}C$ for 45 sec. 3. The results of PRRS antibody test by ELISA method in AI centers were $6.6\%,;53.3\%$ and breeding farms $65\%,;65\%;and;38.7\%$, respectively. The serological positive of the antibody in gilt higher than sow. 4. The sero-positive of the PRRS antibody showed average $21\%$ in domestic farms, $56.2\%$ in breeding farms, and $29.9\%$ in AI center.