The vibration of Powertrain are one of the import design characteristics of a vehicle. Powertrain is mostly mounted to the front subframe and powertrain mounting has an important role in determining the vehicle vibration characteristics. In this paper, the accuracy of the vibration analysis for the front subframe is discussed. The dynamic characteristic of subframe are measured from vehicle test and the finite element model updating are performed that natural frequency, mass and MAC of the experimental and theoretical modal analysis are compared. The subframe mounting stiffness are obtained the iteration method based on the vibration of subframe from vehicle test. Finally, the result of dynamic analysis which is operated dynamic load is compared with experimental one of vehicle test.