One new sterol glucoside, $gomphsterol;{eta}-D-glucoside$ 1 along with known compounds, ${eta}-sitosterol$, stigmasterol, campesterol, $stigmasterol-{eta}-D-glucoside$, friedelin, 3-epi-friedelinol, allantoin, and $chrysoeriol-7-O-{eta}-D-glucoside$ have been isolated from the aerial parts of Gomphrena globosa (Amaranthaceae). On the basis of spectroscopic (including 2D NMR) and chemical studies, the structure of 1 was elucidated as $(22E,24S)-24-ethylcholesta-7,9(11),22-trien-3{eta}-ol-3-O-{eta}-D-glucopyranoside$. Known compounds are reported for the first time from this plant species.