Despite well-known benefits of physical activity for older adults, most older adults remain significantly underactive. The purpose of this study was to examine the effects of a physical activity on blood cholesterol in older adults with an inclusive, choice-based physical activity promotion program to increase lifetime physical activity levels of seniors. A six-month comparison-group trial was conducted with 14 older adults(experimental group = 8, control group = 6) in community senior center. Changes in self-reported physical activity and blood cholesterol were evaluated using paired t-test. The intervention group increased estimated caloric expenditure by 858 calories/week in physical activities of any intensity (p=.050), total cholesterol(p=0.049), high density lipoprotein cholesterol(p=0.045). Control group changes were also negligible. The program led to meaningful physical activity increase. Individually tailored programs to encourage lifestyle changes in seniors may be effective and applicable to health care and community settings.