The purpose of this study was to investigate effects of different chair type (with backrest chair and without backrest chair) and pelvic position (anterior pelvic tilting and posterior pelvic tilting) on three abdominal muscles (upper rectus abdominis, external oblique, internal oblique) and back extensor activation during lower extremity exercise. The four different conditions during bilateral knee extension exercise were: (1) leaning on backrest chair with anterior pelvic tilting, (2) leaning on backrest chair with posterior pelvic tilting, (3) anterior pelvic tilting without backrest chair, and (4) posterior pelvic tilting without backrest chair. Fifteen healthy male subjects with no history of neuromusculoskeletal disease voluntarily participated in this study. Electromyography (EMG) was used to collect muscle activation data, and muscle activation data was expressed as a percentage of maximal voluntary isometric contraction (%MVIC). One-way repeated analysis of variance (ANOVA) was used to determine the statistical significance, and Bonferroni comparison was used as a post hoc test. The results of this study were the following: (1) Erector spinae activation was significantly lower in posterior pelvic tilting without backrest compared with that in leaning on backrest chair with anterior pelvic tilting. (2) Upper rectus abdominis activation was significantly lower than erector spinae in all four different chair type and pelvic tilting conditions.