Flesh wasters from tannery create major environmental problems. Despite their considerable fat content, these waster do not find important usage. Their disposal is also troublesome and costly. We have investigated the possible use of this fat as the production of biodiesel(fatty acid methyl esters) by transesterification using with fossil fuels. The fat released the waste by boiling water under the optimal condition (i.e., temperature, $120^{circ}C;$ decompression, 200mbar) and used to dry without refining for the production of fatty acid methyl esters. Under the optimal condition, the experimental value of biodiesel yield was about 96%. The result of the chemical and GC analysis showed fatty acid composition and characteristics of biodiesel. Evaluation of the product indicated that it was suitable for use as a biodiesel fuel. In result of this experiment oil extract from fleshing process can be transformed into an environmentally affination fuel, to provide economical and ecological profits.