This study was conducted to investigate the effect of soil, cultural practices and climatic conditions on some chemical constituents of flue-cured tobacco. Increasing the nicotine and total nitrogen contents may be useful to reduce the total sugar content of cured leaves in flue-cured tobacco. Delaying the transplanting date and increasing the soil nitrogen($N0_3-N$ and $N0_4-N$) content for 30 days after transplanting by fertilizing are desirable so as to increase the nicotine and total nitrogen contents of cured leaves. Those treatments will delay the ripeness, and elongate the duration of cultivation(day from transplanting to harvesting), and increase the fresh leaf weight. Moderate rainfall in April and May, lower relative humidity in June, and higher mean daily air temperature in June and July seem to be necessary for good leaf of flue-cured tobacco in Korea.