An experiment was conducted to know the essential oil penetration depth in radial and longitudinal direction of Prunus sargentii. Oil penetration depth was found greater than radial flow depth. Vessel conducted oil more than wood fiber. In radial direction, body ray parenchyma was found more permeable than marginal ray parenchyma and it was about 138% times higher. Furthermore penetration depth of oil in intercellular space was greater than ray parenchyma and it was about 250% higher than ray parenchymas. Initial flow speed was found high and then it gradually decreased.