A study was conducted to know 99.5% ethyl alcohol penetration depth in radial and longitudinal direction of Castanea crenata. Alcohol penetration depth was found higher in longitudinal direction by about 12.47 times. In both earlywood and latewood, fiber conducted alcohol in higher depth than that of large and small vessel. Penetration depth of alcohol after 15.0 second of penetration in longitudinal direction, there was no significant difference among fiber in sapwood and heartwood, and vessel in sapwood. At the beginning of penetration the speed was high and then gradually decreased.