An elbow orthosis with a pneumatic rubber actuator has been developed to assist and enhance upper limb movements and has been examined for the effectiveness. The effectiveness of the elbow orthosis was examined by comparing muscular activities during alternate dumbbell curl exercises with and without the orthosis. The subjects participated in the experiment were younger adults in their twenties. The subjects were instructed to perform dumbbell curl motion in a sitting position with and without orthosis in turn and a dynamometer was used to measure elbow joint torque outputs in an isovelocity mode. The measurements were done with four various dumbbell loads: 0 kg, 1 kg, 3 kg, and 5 kg. The orthosis was pneumatically actuated and controlled in a passive mode. The most comfortable air pressure to the pneumatic actuator was determined to be 0.294MPa. Electromyography(EMG) was also measured during curl exercises. The muscles of interest were biceps brachii(BB), triceps brachii(TB), brachioradialis(Bo), and flexor carpi ulnaris(FCU) in the upper limbs. The experimental results showed that the muscular activities themselves significantly reduced with elbow orthosis on in performing similar activities without orthosis. As a result of this experiment, the effectiveness of the developed upper limb orthosis was confirmed and the level of assistance was quantified.