In this study, a new bicycle system was developed to improve muscular strength using the Magneto-Rheological(MR) rotary brake. The friction load of the MR rotary brake is adjusted according to muscle strength of the subjects. The characteristic of muscular strength was studied with various friction loads of MR rotary brake. The friction load was occurred with the current, applied to the MR. rotary brake. Experiments was composed of several cycling trials for various friction loads. In training programs involving muscle improvement, it is necessary to confirm muscle activity and fatigue. To measure the muscle activity and fatigue, EMG signals of rectus femoris (RF), biceps femoris (BF), tensor fasciae latae (TFL), vastus lateralis (VL), vastus medialis (VAS), gastrocnemius (GAS), tibialis anterior (TA) and soleus (SOL) muscles were collected with surface electromyography and analyzed into time and frequency domain. The experimental results showed that the muscle activity according to the applied current to the MR rotary brake was significantly different. The more the current was applied, the higher value of the integrated EMG (IEMG) was obtained. Especially, the magnitude of IEMG of the RF, BF, TFL and VL varied in direct proportion to the current. However, there was not significant in the median frequency as the cycling time continue.