Objectives: The research was conducted to evaluate the socio-demographic, life style and work related factors of stress and fatigue, and correlation between them in the white collar workers. Methods: This study was targeted on white collar workers of medium-sized company in Kimhae city including 249 people; 167 of males, 82 of females. The data was collected by structured questionnaires and analyzed using t-test, ANOVA, Pearson correlation and stepwise multiple regression with SPSS 12.0. Result: Both stress and fatigue levels were higher for female of gender, 20s of age, single of marital status in socio-demographic factors. The stress level was higher for current smokers and lower frequency of regular exercise per week, and both stress and fatigue levels were higher for unsatisfied sleeping in lifestyle factors. The stress level was lower for 5-day workweek system and experience of downsizing, and both stress and fatigue levels were higher far lower period of employment in work related factors. The correlation coefficient(r) between stress and fatigue level was 0.578, which was highly statistically significant. According to stepwise multiple regression analysis, factors affecting stress were unsatisfied sleeping, female and lower frequency of regular exercise per week, and those affecting fatigue were younger age and unsatisfied sleeping. Conclusion: This study provided strong support for associations between stress and fatigue, and we suggest that modifiable factors such as regular exercise and satisfied sleeping would be important for white collar workers to reduce their stress and fatigue.