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Protein Profiles of Major Korean Rice Cultivars
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  • Protein Profiles of Major Korean Rice Cultivars
  • Protein Profiles of Major Korean Rice Cultivars
저자명
Wei. Chuanha,Kwon. Oh-Yun,Liu. Xi-Wen,Kim. Hyoung-Chin,Yoon. Won-Kee,Kim. Hwan-Mook,Kim. Mee-Ree
간행물명
Journal of food science and nutrition
권/호정보
2007년|12권 2호|pp.103-110 (8 pages)
발행정보
한국식품영양과학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

The protein profiles among Korean rice cultivars were assessed by total protein determination, solubility fractionation, SDS-PAGE analysis and scanning densitometry. In the extraction of protein, the SDS/urea system at a neutral pH was more efficient than that at alkaline pH. The determination of total protein showed that the protein content was similar among cultivars, ranging from 87.9 to 92.7 mg/g dry weight. Additionally, the water/NaCl-soluble protein fraction, containing 14${sim}$16 kDa albumin and 22 kDa globulin ${alpha}$-globulin, was also similar among cultivars, with a range of 9.94 to 11.98 mg/g dry weight. The SDS-PAGE/densitometry of total protein showed that there was no discernable difference in proteins of higher molecular weights among various cultivars, whereas the amount of lower molecular weight proteins (14${sim}$16 kDa) is somewhat variable among cultivars. Furthermore, SDS-PAGE analysis of water/NaCl-soluble and propanol-soluble fractions indicates that there is a discernible change in the content of albumin, globulin or prolamin among cultivars. Thus, the PAGE/densitometry method, preceded by solubility fractionation, is useful for examining differences in protein profiles of rice cultivars.