Heterotrimeric GTP binding proteins, G-proteins, mediate signal transduction generated by neurotransmitters and hormones. Among G-proteins, Go proteins are the most abundant in brain and classified as a member of Gi family. Ras-like protein in all tissues (Rit), one of the small GTPases, is a member of a Ras superfamily and identified as an important regulator of neuronal differentiation and cell transformation. Recently, we have reported that Rit functioned as a candidate downstream effector for alpha subunit of Go proteins ($Go{alpha}$) and regulated neurite outgrowth triggered by $Go{alpha}$ activation. In this study, we showed that the GTPase domain of $Go{alpha}$ contributed to the direct interaction with Rit. We also demonstrated that $Go{alpha}$ could lead to an increase of Rit activity suggesting that Rit play a role as a downstream effector of $Go{alpha}$.