The purpose of this study is to determine the effect of chitosan treatment on the bamboo charcoal dyed cotton knit. The K/S, CIE $L^*$, $a^*$, $b^*$ and Munsell values of dyed and chitosan treated samples were measured. As chitosan treatment concentration increased, dye uptake decreased progressively. The chitosan treated cotton knit showed a red tone and a yellow tone on the Munsell color system. The antibacterial property, deodorization performance, moisture regain, thermal transmittance property were improved by chitosan treatment. The bamboo charcoal dyed and chitosan treated cotton knit showed high colorfastness to dry cleaning and washing.