Chromatographic separation of the EtOAc fraction from the leaf and stem of Vitis amurensis led to the isolation of six oligostilbenoids (i.e., r-2-viniferin (1), trans-amurensin B (2), trans-$varepsilon$-viniferin (3), gnetin H (4), amurensin G (5), (+)-ampelopsin A (8)) and four stilbenoids (i.e., trans-resveratrol (6), (+)-ampelopsin F (7), piceatannol (9), and trans-piceid (10)). The structures have been identified on the basis of spectroscopic evidence and physicochemical properties. The isolates were investigated for cytotoxic activity against three cancer cell lines in vitro using the MTT assay method. Amurensin G (5) and trans-resveratrol (6) showed significant cytotoxic activity against L1210, K562 and HTC116 cancer cell lines with $IC_{50}$ values ranging from $15.7;{pm};2.1$ to $30.9;{pm};1.8;{mu}M$. (+)-Ampelopsin A (8) and trans-piceid (10) exhibited considerable cytotoxic activity against L1210 ($IC_{50}$ values of $30.6;{pm};4.1$ and $28.7;{pm};2.81;{mu}M$, respectively) and K562 ($IC_{50}$ values of $38.6;{pm};0.82$ and $24.6;{pm};0.76;{mu}M$, respectively). Gnetin H (4) showed only weak cytotoxic activity against L1210 with an $IC_{50}$ value of $40.1;{pm};4.23;{mu}M$. On the other hand, r-2-viniverin (1), trans-amurensin B (2), trans-$varepsilon$-viniferin (3), (+)-ampelopsin F (7), and piceatannol (9) exhibited no activity on three cancer cell lines.