The spray characteristics of a 6-hole injector were examined in a single cylinder optical direct injection spark ignition engine. The effects of injection timing, in-cylinder charge motion, fuel injection pressure, and coolant temperature were investigated using the 2-dimensional Mie scattering technique. It was confirmed that the in-cylinder charge motion played a major role in the fuel spray distribution during the induction stroke while injection timing had to be carefully considered at high injection pressures during the compression stroke to prevent spray impingement on the piston.