In this paper, the influence of injection parameters on the transition from Premixed Charge Combustion Ignition (PCCI) combustion to conventional diesel combustion was investigated in an optically accessible High-Speed Direct-Injection (HSDI) diesel engine using multiple injection strategies. The heat release characteristics were analyzed using in-cylinder pressure for different operating conditions. The whole cycle combustion process was visualized with a high-speed video camera by simultaneously capturing the natural flame luminosity from both the bottom of the optical piston and the side window, showing the three dimensional combustion structure within the combustion chamber. Eight operating conditions were selected to address the influences of injection pressure, injection timing, and fuel quantity of the first injection on the development of second injection combustion. For some cases with early first injection timing and a small fuel quantity, no liquid fuel is found when luminous flame points appear, which shows that premixed combustion occurs for these cases. However, with the increase of first injection fuel quantity and retardation of the first injection timing, the combustion mode transitions from PCCI combustion to diffusion flame combustion, with liquid fuel being injected into the hot flame. The observed combustion phenomena are mainly determined by the ambient temperature and pressure at the start of the second injection event. The start-of-injection ambient conditions are greatly influenced by the first injection timing, fuel quantity, and injection pressure. Small fuel quantity and early injection timing of the first injection event and high injection pressure are preferable for low sooting combustion.