Purpose: This research was conducted to determine the effects on HPV knowledge, cervical cancer preventive behaviors, and sexual autonomy of female university students after they received sexual education to prevent HPV involved cervical cancer. Method: By using a non-equivalent control group pretest and posttest design, the experiment was conducted with a test group of 37 students and a control group of 39 students. Result: There were statistically significant increases in HPV knowledge (p=.003), cervical cancer preventive behaviors (p=.001), and sexual autonomy (p=.005) in the experimental group compared to the control group. Conclusion: It is concluded that this research is valid because this research program has proved to be effective in increasing HPV knowledge, cervical cancer preventive behaviors, and sexual autonomy, and it has provided a foundation to be used with other female university students.