Enhancement of ${gamma}$-Amminobutyric Acid Production by Lactobacillus sakei B2-16 Expressing Glutamate Decarboxylase from Lactobacillus plantarum ATCC 14917

Enhancement of ${gamma}$-Amminobutyric Acid Production by Lactobacillus sakei B2-16 Expressing Glutamate Decarboxylase from Lactobacillus plantarum ATCC 14917

ㆍ 저자명: Kook. Moo-Chang,Seo. Myung-Ji,Cheigh. Chan-Ick,Lee. Sang-Jae,Pyun. Yu-Ryang,Park. Hoon
ㆍ 간행물명: Journal of the Korean Society for Applied Biological Chemistry
ㆍ 권/호정보: 2010년|53권 6호|pp.816-820 (5 pages)
ㆍ 발행정보: 한국응용생명화학회
ㆍ 파일정보: 정기간행물|
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ㆍ 주제분야: 기타

이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.

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기타언어초록

To enhance ${gamma}$-amminobutyric acid (GABA) production using genetically engineered lactic acid bacteria, glutamate decarboxylase gene was isolated from Lactobacillus plant arum ATCC 14917 and cloned into Lactobacilli-E. coli shuttle-expression vector (pTRKH2) to yield pTRKH2GAD, which was expressed in L. sakei B2-16 isolated from Kimchi. The recombinant L. sakei B2-16 showed 1.35- and 1.42-fold higher GABA production compared to the untransformed strain in 500-mL flask and 5-L fermentor scale, respectively. The maximum GABA concentration was 265.3 mM.

키워드

${gamma}$-amminobutyric acid glutamate decarboxylase Lactobacillus plantarum Lactobacilus sakei

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