An inter-propellant seal (IPS) for 75 ton class thrust turbopump was tested. Leakage characteristics were presented with a given range of pressure difference under cryogenic as well as room temperature conditions. For cryogenic tests, liquid nitrogen was used as analogic fluid of liquid oxygen (LOX) while water was used instead of kerosene for room temperature condition. Test results showed that IPS had satisfactory leakage performance. Additionally endurance test was conducted to prove the life time of manufactured IPS and the tested IPS had successfully survived during required life time, 2100 seconds.