Purpose: The purpose of this study was to examine symptoms, mood and sleep disturbance in patients with hemodialysis. Method: A sample of 96 hemodialysis patients participated. Data were collected using symptom scale of hemodialysis patients, profile of mood state and the sleep scale. Statistical analytic methods included t-tes, ANOVA and Pearson correlation analyses. Results: Patients with spouse/partners experienced significantly less symptoms than those with no spouse (F=6.29, p=.003). Mood disturbance was not significantly correlated with age; but older patients experienced higher sleep disturbance (F=4.88, p=.010). Symptoms, mood and sleep disturbance are significantly related with each other. Conclusion: It is important to assess symptoms, mood and sleep disturbance in older hemodialysis patients with no spouse. It is needed to repeat extensive study and comparative study with other population in order to define clearly.