Trans fatty acids (t-FA) contents were analyzed in 26 food items that were collected from Korean grocery stores in 2005 and 2008. Lipid was extracted through Soxhlet and Mojonnier methods. Fatty acid profile, including t-FA and positional fatty acid composition was analyzed by gas chromatography. The comparative study of fatty acids composition including t-FA was employed in 2005 and 2008. Among the analyzed food items, most of the food items showed higher lipid content in 2005 than in 2008. Some food items such as biscuits (0.4-7.2 g/100 g of food), fried snacks (0.1-3.6 g/100 g of food), and cookies (0.4-2.4 g/100 g of food) contained relatively higher level of t-FA than other food groups (cracker, processed chocolate, and ice cream) in 2005. Comparing t-FA content in 2005, it was considerably decreased up to 91.5% in 2008 whereas saturated fatty acids (SFA) were increased up to 43.3% in the analyzed food items.