The aim of this study was to investigate the effects of persimmon-vinegar supplementation on blood lipid profiles, carnitine concentrations, and hepatic mRNA levels of enzymes involved in fatty acid metabolism. Thirty-two C57BL/6J male mice were divided into 4 groups; control group (HD), industrial vinegar group (HDV), and persimmon-vinegar groups (HD-PV1, HD-PV2). Serum triglyceride (TG) and total cholesterol (TC) concentrations significantly decreased in all vinegar-administered groups compared with the HD group. The hepatic TG and TC concentrations of persimmon-vinegar administered groups were significantly lower compared with the HD group. Liver acid insoluble acylcarnitine (AIAC) was significantly higher in the HD-PV2 than in HD and HD-V groups. The acetyl-CoA carboxylase (ACC) mRNA level tended to lower in all the vinegar administered groups compared with the HD group. These results suggest that the persimmon-vinegar has anti-obesity properties.