To investigate effects of pine essential oil isolated by steam distillation from Pinus densiflora needles (PN) and twigs (PT) on anti-oxidant and anti-aging activities, the experiments were carried out to determine anti-oxidant and antiaging activities on DPPH radical scavenging activity, NBT/xanthine oxidase-superoxide scavenging activity, silica-induced intracellular $H_2O_2$ and hydroxyl radical generation in RAW264.7 cells, hyaluronidase and elastase activities in vitro. Essential oil of PN and PT were contained 0.225 and 0.176 (w/v) %, respectively. PN was contained with 30 kinds of essential oil and its major constituent is $alpha$-pinene (21.5%). Further PT was contained with 40 kinds of essential oil and its major constituent is $eta$-pinene (22.4%) in GC/MS assay. Other essential oils of PN were $eta$-pinene, camphene, myrcene, limonene, terpinolene, $alpha$-terpineol, bornyl acetate, $alpha$-copaene, caryophellene and humulene and PT were $alpha$-pinene, camphene, phellandrene, limonene, terpinolene, $alpha$-terpineol, bornyl acetate, $alpha$-copaene, caryophellene and humulene. The essential oil of PT have more active than that of PN in anti-oxidant activity which has significant DPPH radical and superoxide scavenging activity, and significant inhibitory activities on silica-induced intracellular $H_2O_2$ and hydroxyl radical generation, as well as, significantly inhibited elastase and hyaluronidase activities. Further, phellandrene, myrcene and $alpha$-pinene have DPPH radical and superoxide scavenging activities, $eta$-pinene, terpinolene, myrcene and phellandrene inhibited silica-induced intracellular $H_2O_2$ and hydroxyl radical generations. And also phellandrene and $eta$-pinene inhibited hyaluronidase and elastase activities. In conclusion, the essential oils isolated from PN and PT have anti-oxidant and anti-aging activities.