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타액 중 ${Delta}^9$-Tetrahydrocannabinol 및 11-Nor-9-carboxy-${Delta}^9$-Tetrahydrocannabinol의 분석법 확립 및 안정성 검토
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  • 타액 중 ${Delta}^9$-Tetrahydrocannabinol 및 11-Nor-9-carboxy-${Delta}^9$-Tetrahydrocannabinol의 분석법 확립 및 안정성 검토
  • Development of Quantification Method and Stability of ${Delta}^9$-Tetrahydrocannabinol and 11-Nor-9-carboxy-${Delta}^9$-Tetrahydrocannabinol in Oral Fluid
저자명
최혜영,백승경,장문희,최화경,정희선,Choi. Hye-Young,Baeck. Seung-Kyung,Jang. Moon-Hee,Choi. Hwa-Kyung,Chung. Hee-Sun
간행물명
약학회지
권/호정보
2010년|54권 4호|pp.226-231 (6 pages)
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

Oral fluid has become increasingly popular as an alternative specimen in the field of driving under the influence of drugs (DUID) and work place drug testing. In this study, an analytical method for the detection and quantification of ${Delta}^9$-tetrahydrocannabinol (THC) and its metabolite, 11-nor-9-carboxy-${Delta}^9$-tetrahydrocannabinol (THC-COOH) in oral fluid by SPE and GC-MS was established and fully validated. The stability of THC and THC-COOH in oral fluid during storage was also determined by examining the THC and THC-COOH concentration changes depending on time and container materials. Oral fluid samples were kept over 21 days at room temperature, $-4^{circ}C$ and $-20^{circ}C$ in two different specimen collection tubes; glass and polypropylene tubes. Three replicates for each condition with different temperature and types of a container were analyzed at five different time points over 21 days. When oral fluid samples were stored in glass tubes, the loss of both THC and THC-COOH was less than 10% at all room temperature, $-4^{circ}C$ and $-20^{circ}C$. However, in polypropylene tubes, the loss of both THC and THC-COOH increased significantly over the study period. In particular, the concentration of THC decreased more rapidly than that of THC-COOH at room temperature and the maximal percentage of THC lost was 90.3% after 21 days. The result indicates that it would be necessary to collect oral fluid samples in glass containers and cool the samples until analysis in order to prevent the degradation of analytes.