The enzymatic processing conditions of chitosan nonwoven using cellulase and papain were optimized to determine the correlation between chitosan nonwoven and both enzymes. The hydrolytic activity and structural change in the chitosan fiber were evaluated. Chitosan hydrolysis by cellulase was maximized using 10% (o.w.f.) cellulase at $40^{circ}C$ for 30 minutes under pH 5, whereas that by papain was optimized at a papain concentration of 10% (o.w.f.), and $60^{circ}C$ for 60 minutes at pH 8. The degree of deacetylation of the chitosan decreased by 3.9% and 2.4% using cellulase and papain, respectively, which reduced slightly the level of acid dye adsorption of chitosan. DSC, XRD, FT-IR and GPC analysis confirmed no substantial changes in the molecular and crystalline structures of chitosan by the enzymatic treatment.